Real-time Ultra-large-Scale High-resolution macroscopy (RUSH)

Summary

Real-time Ultra-large-Scale High-resolution macroscopy (RUSH) is the largest field-of-view high resolution microscopy in the world. It is designed for various applications in life science.

Technical Specifications

  • FOV:1.0 cm*1.2 cm
  • Resolution: 800 nm
  • Frame rate: 30 Hz
  • Data Throughput: 11.55 GB/s

Motivation

Large-scale imaging of biological dynamics at high resolution in vivo is fundamental for systems biology. For example, large-scale recording of neural network activity in vivo, is set as one of the major goals of both BRAIN Initiative and Chinese Brain Project.

However, conventional microscopes could not complete the requirements of both high optical resolution and large field-of-view (FoV) simultaneously. And recent reports on mesoscopes are still of low data throughput..

Our innovations:

  • A novel objective at cm scale FoV and sub-μm resolution
  • A high-throughput camera array of 35 sCMOS for image sensing

Performance

Real-time, Ultra-large-Scale imaging at High resolution (RUSH) macroscope:

  • FOV:1.0 cm*1.2 cm
  • Resolution: 800 nm
  • 169 million pixels per frame
  • Frame rate: 30 Hz
  • Data Throughput: 11.55 GB/s
The largest FoV, highest throughput macroscope at high resolution at the moment.

Experiment results

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